![]() ![]() Appropriate wash buffer: Phosphate-buffered saline (PBST): 10 mM sodium phosphate, pH 7.2, 0.9% (w/v) NaCl, up to 0.1% Tween-20 detergent, TBST: 10 mM Tris, pH 7.4, 0.9% (w/v) NaCl, up to 0.1% Tween-20.Substrate appropriate to the enzyme conjugate (HRP or AP).Secondary antibody (specific for primary antibody), labeled with alkaline phosphatase or horseradish peroxidase.Primary antibody (specific for protein of interest).The advantage is that standard immunodetection may require less optimization for new sample types. The drawbacks of this method are the need for blocking and the total time requirement of over 4 hours. The unoccupied membrane binding sites on the wet blot are blocked with optimized reagents. (If the membrane was dried after transfer, thoroughly wet the blot for 1 minute in methanol if using PVDF or Milli-Q water if using nitrocellulose before proceeding to immunodetection.) Standard immunodetection is performed on blotted proteins directly after electrotransfer. Standard immunodetection, however, offers higher sensitivity and requires less optimization for new sample types. The rapid immunodetection method works well to quickly visualize higher abundance proteins. Rapid immunodetection eliminates the blocking step and reduces the time necessary for the washing and incubation steps. Incubating the membrane with a substrate that reacts with the conjugated secondary antibody to reveal the location of the protein.Washing to remove any unbound secondary antibody.Incubating the membrane with a conjugated secondary antibody, which binds the first antibody.Washing to remove any unbound primary antibody.Incubating the membrane with primary antibody, which binds to the protein of interest.Blocking unoccupied membrane sites to prevent nonspecific binding of antibodies.Standard Immunodetection Methods Include the Following Steps: There are two types of protocols for immunodetection: Standard and rapid. If the specific antibody concentration of a given unpurified antibody preparation is unknown, we recommend using a concentration/purification kit and refer to the table below as a guideline.New protocol saves time and delivers consistency. Unpurified antibody preparations differ significantly in antibody concentration. Suggested dilutions for antibodies with no recommended dilution on the datasheet However, especially for polyclonal antibodies, when there is a change in the staining results between batches of the same antibody, we recommend performing another titration experiment. Many antibodies will have similar batch-to-batch consistency therefore, only one titration experiment is required in most cases. Each dilution should be tested on the same sample type to keep the same experimental conditions. For example, if a product datasheet suggests using a 1:200 dilution, it is recommended to make dilutions of 1:50, 1:100, 1:200, 1:400 and 1:500.Ī titration experiment is done by first selecting a fixed incubation time and then a series of experimental dilutions of the antibody. The optimal antibody concentration, which gives the best staining with minimum background, must be determined experimentally for each assay and is usually determined using a series of dilutions in a titration experiment. Optimizing the antibody dilution: titration experiments However, they may require some optimization in your specific experimental setup. Usually, antibodies have recommended dilutions for various applications included in the datasheet. As it is not usually possible to change the antigen concentration, the optimal working concentration of each antibody must be determined with dilutions for each application and set of experimental conditions. Varying the relative concentrations of an antibody and an antigen solution can also control the extent of antibody-antigen complex formation. The rate of binding between antibody and antigen – affinity constant – can be affected by temperature, pH, and buffer constituents. Why do you need to define antibody dilution for your experimental conditions? ![]()
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![]() ![]() The early architecture of Uber consisted of a monolithic backend application written in Python that used Postgres for data persistence. Fun fact - earlier in Uber's history we'd actually moved from MySQL to Postgres before switching back for good, & though we published the article in Summer 2016 we haven't looked back since: In essence, it was due to a variety of limitations of Postgres at the time. Our most popular (& controversial!) article to date on the Uber Engineering blog in 3+ yrs. We use its excellent built-in full-text search, which has helped us avoid needing to bring in a tool like Elasticsearch, and we've really enjoyed features like its partial indexes, which saved us a lot of work adding unnecessary extra tables to get good performance for things like our "unread messages" and "starred messages" indexes. ![]() As a result, we were able to delete a bunch of custom queries escaping the ORM that we'd written to make the MySQL query planner happy (because postgres just did the right thing automatically).Īnd then after that, we've just gotten a ton of value out of postgres. We didn't have to do any real customization (just some tuning settings for how big a server we had), and all of our most important queries were faster out of the box. We ended up getting so frustrated that we tried out PostgresQL, and the results were fantastic. ![]() Issues ranged from bad collation defaults, to bad query plans which required a lot of manual query tweaks. However, we found that even though we were using the Django ORM for most of our database access, we spent a lot of time fighting with MySQL. Zulip started out as a MySQL project back in 2012, because we'd heard it was a good choice for a startup with a wide community. We've been using PostgreSQL since the very early days of Zulip, but we actually didn't use it from the beginning. In the end, we got to implement a highly scalable near realtime Change Data Replication service that "works" and deployed to production in a matter of few days! See more With Zappa, deploying your services as event-driven & horizontally scalable Lambda service is dumb-easy. We deployed this micro-service as AWS Lambda with Zappa. We implemented source data to target data translation by modelling target table structures through SQLAlchemy. Next we wrote a minimal micro-service in Python to listen to the message events on SQS, pickup the data payload & mirror the DB changes on to the target Data warehouse. In the Node.js function, we wrote minimal functionality to communicate the database changes (insert / update / delete / replace) to Amazon SQS. Interestingly enough, MongoDB stitch offers integration with AWS services. We chose Amazon SQS as the pipe / message backbone for communicating the changes from MongoDB to our own replication service. When there are a lot of database changes, Stitch automatically "feeds forward" these changes through an asynchronous queue. Using stitch triggers, you can execute a serverless function (in Node.js) in real time in response to changes in the database. One of the services offered by MongoDB Stitch is Stitch Triggers. It is the serverless platform from MongoDB. ![]() We chose MongoDB Stitch for picking up the changes in the source database. The data replication must be horizontally scalable (based on the load), asynchronous & crash-resilientīased on the above criteria, we selected the following tools to perform the end to end data replication: The data replication must be near real-time, yet it should NOT impact the production database We set ourselves the following criteria for the optimal tool that would do this job: Recently we were looking at a few robust and cost-effective ways of replicating the data that resides in our production MongoDB to a PostgreSQL database for data warehousing and business intelligence. ![]() ![]() ![]() Work on the National Grid maps stopped for the duration of the war. However, it had not become established when World War II broke out and the Cassini Grid continued in use throughout the war. The Ordnance Survey National Grid was introduced in 1938 and the intention was to replace the Cassini Grid system with this. However, it is frequently not necessary to explicitly give the grid square at all, this can be implied from context. This is because square qY is three 100 km squares East of square vV and five 100 km squares North of vV. For instance qY 985 613 (somewhere around Carlisle) can also be expressed as 3985 5613. To do this, square vV (the South-West corner of the grid out in the Atlantic) is taken as square zero and then the grid squares are counted from there to the location being referenced. Grid references can also be given in an all numeral notation. For instance, wQ 683 825 is a location in South London. The complete grid reference includes both the grid square letters and the numerical reference giving the location within the grid square. Greater precision can be obtained by using six, eight, or even more digits, but always an even number with the same precision on both coordinates. That is, it is two squares across and seven squares up (counting from zero) from the reference corner. For instance, the grid reference 25 76 refers to a 1 km square within the 10 km square 2 7. These squares are numbered according to eastings and northings, that is, counting from the south-west corner of the 100 km square. Įach 100 km square is subdivided into 10 km squares, and each of these is further subdivided into 1 km squares. For instance, the squares covering London are wL and wQ. The full designation for a grid square is the 500 km letter in lowercase followed by the 100 km letter in uppercase. The 100 km squares are also given letters (with I omitted). Each 500 km square is further divided into 25 100 km squares. Ireland is covered by square I, but the Irish grid is tilted slightly to the East relative to the English grid. The squares covering England are Q, R, V and W with an intersection somewhere between York and Thirsk. The map is divided into a grid of 500 km squares. Hong Kong, for instance, was surveyed in 1962 for a series of 1:10,000 and 1:25,000 maps of the territory. However, the Cassini Grid system continued to be used to map colonial possessions into the 1960s. Īfter the war, the Cassini Grid system was replaced with the Ordnance Survey National Grid (see § Transition to National Grid). It continued in use until some time after World War II. It may also be referred to as Military Grid, War Office Grid, or Purple Grid (due to the grid colour on some issues of maps). The Cassini Grid system is thus more properly called the Modified British System. It modified and replaced a grid coordinate system first deployed in 1919 known as the British System. It is so called from the use of Cassini map projection. The Cassini Grid system was introduced in 1927 for maps of the United Kingdom for the British military. The system has been superseded by the Ordnance Survey National Grid. For a period after the war, the maps were also used by the general public. The referencing consists of square grids drawn on a Cassini projection. The Cassini Grid was a grid coordinate system used on British military maps during the first half of the twentieth century, particularly during World War II. This tool is perfect for locating precise geographical details for any location within Great Britain.Coordinate system used on British military maps These details can be found below the map. These coordinates include the latitude and longitude (in decimal degrees), the Ordnance Survey (OS) six-figure grid reference, and the OS grid square letter reference. Upon clicking, a marker will appear on the clicked location, and the corresponding geographical coordinates will be displayed on the screen. To pinpoint a specific location and get its geographical information, simply click anywhere on the map. You can also navigate around the map by dragging it with your mouse. Once you've entered your desired location, the map will automatically zoom in to the area. Begin by searching for a postcode or place in the search bar located at the top right corner of the map. Welcome to our interactive map! This tool allows you to find specific geographical coordinates for any location within Great Britain, based on your input. ![]() ![]() ![]() ![]() You're talking about a lot you don't know about here. Look up the project by John Lin on Youtube as well, that's fully software raytraced rendered, including raytraced audio Teardown is entirely fully raytraced rendered in software, as in does not use RTX hardware or cores and if you were to try to disable raytracing at all you'd get a black screen, nothing would render, and will run on 10 series and pre 16xx series AMD GPU's. It may not be ready for wide adoption up and down the budget stack, but the future is bright and it's the correct direction to move in strategically. You can argue against this early iteration of the technology for not being as fast as the tricks that have been put in place for rasterization, but the benefits on both the consumer side and engineering side are REAL. Real time RT is quite literally the biggest leap forward for both rendering accuracy and developer workflow in a long time. RT is the future, but it's still EARLY days as well as an extremely heavy computation to do in real time. turning rtx on and off on quake 2 rtx is like night and day. Originally posted by Rongkong Coma:Why is everyone so ♥♥♥♥♥♥ up because of this ray tracing ♥♥♥♥?! The Quake 2 and 3 soundtracks are also listed here as Public Domain which is highly unlikely in my opinion. In fact - there's been an official vinyl release of this soundtrack in 2020, so this makes it even less probably in m eyes. is the most worse fact why I am frustrated about.ĭont know why everyone wines about raytracing when the basics given are absolutely catastrophic!? :-( I cannot find any information about this being the case for Quake I soundtrack. especially because of the crossplay.īut with this missing it will stay like quake champions. an ingamebrowser for user-generated content like singleplayermaps or Mulitplayermaps. Much worse I think is the fact that Nightdive did not put an overworked editor into the new game and. Quake is oldschool and that is what the game should stay. Quake II is a first-person shooter, in which the player shoots enemies from the perspective of the main character.The gameplay is very similar to that featured in Quake, in terms of movement and controls, although the players movement speed has been slowed down, and the player now has the ability to crouch. the games would run so far on a RTX 3090 only in 1024x768 resolution with 60 FPS.įor real raytraing we have to wait 5 years more and roundabout 3 Graphic-Generation-Cards to get into a real taste of pure raytracing.Īnd the retrofactor would be completely killed if raytracing would be in Quake 1 remaster. and I mean if they REALLY put very much raytracing effects in games. see The Ascent and so on.Īnd if the programmer would do very much effects into a game to use completely raytracing. The effects are minimal so far in the games. Ray tracing is the most uninterresting thing ever in Games history. Why is everyone so ♥♥♥♥♥♥ up because of this ray tracing ♥♥♥♥?! ![]() ![]() ![]() ![]() ago Its probably the best stuff pack, as far as actual stuff goes. 3 6 6 comments Best Add a Comment whyarepangolins 2 yr. NVIDIA GeForce 6600, ATI Radeon X1300, Intel GMA X4500ġ. Is paranormal stuff fun or a waste of Do you guys like paranormal stuff Is it worth it Waiting on a farm pack sigh one day. OS:Ħ4-bit Windows 7, Windows 8.1 or Windows 10ġ.8 GHz Intel Core 2 Duo, AMD Athlon 64 Dual-Core 4000+ With spooky activity on the rise, it’s up to your Sim to put a stop to it.īase game (The Sims 4) required in order to play. Calm the Spirits - Master the Medium skill and impress Guidry to get a Paranormal Investigator license! Once you’re ready, take on some freelance gigs to expel unwelcome visitors for other Sims.Remove accursed objects, perform a séance, commune with the dead, and receive gifts from specters! You can even summon Bonehilda the skeletal maid. 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The Sims 4 Paranormal stuff releases soon, and its easy to see its similarities to the Ghost Hunter profession of The Sims 3s Ambitions expansion. ![]() When will we see her make an appearance in The Sims 4 It was recently announced that the Paranormal Stuff Pack will be released on January 26th Advertisement. She first arrived in The Sims 1 and returned in The Sims 3. ATTENTION! Only suitable for EUR accounts. Bonehilda has been missed by many, the skeletal maid was. Required ⓘ This product only works on New Accounts!īy completing this purchase, I confirm that I have read and understood the product description.Īttention! Only suitable for NL accounts. ONLINE REQUIREMENTS: Internet connection required for product activation. VIDEO: NVIDIA GeForce GTX 680, AMD Radeon RX 560 or better The Sims 4 has announced the release date for the games horse-themed expansion, while debuting a trailer. HARD DRIVE: At least 15 GB of free space with at least 1 GB additional space for custom content and saved games Mac OS® X 10.11 (El Capitan)ĬPU: Intel Core 2 Duo 2.4GHz Processor or better Minimum THE SIMS 4: PARANORMAL STUFF system requirements for PC Our Easy to follow 3-step purchase system contains no annoying forms or surveys to fill out and only requires an email address and a valid payment method, thus making the process of buying THE SIMS 4: PARANORMAL STUFF DLC for PC from quick and easy. Our Live Chat (24/7) and excellent customer support are always available in case you have any trouble or questions regarding the THE SIMS 4: PARANORMAL STUFF DLC for PC code. (pre-order products will be delivered before or on the release date mentioned) ![]() Once purchased, we will send you the THE SIMS 4: PARANORMAL STUFF DLC for PC digital code instantly and directly to your provided email address. Besides being cheap, you can be rest assured our codes are 100% legit as they are bought from official suppliers. Our cheap prices are because we purchase digital codes in bulk at a discounted rate that we in turn pass onto you, our customers. We have some of the cheapest THE SIMS 4: PARANORMAL STUFF DLC for PC codes on the market. Note: THIS IS DOWNLOADABLE CONTENT OR DLC, ORIGINAL GAME IS REQUIRED TO PLAY THIS EXPANSION. In order to make sure you can still get the most out of it we've updated our haunted house guide so you too can survive the night and reap the rewards. ![]() ![]() In early July 2008, Samuel Alito stood on a riverbank in a remote corner of Alaska. ![]() In 2020, a white security guard in Oklahoma who professed his support for former President Donald Trump and resigned from the Tulsa County Sheriff's Office after being disciplined, shot and killed a Black man in a hotel parking lot ahead of the former president's rally in the area.Ĭlarence Thomas’ Beneficial Friendship With a GOP Megadonor Private security guards, much like city and county law enforcement, have often been at the center of controversy. The female was then asked to leave and the female left (the) location.'"Īccording to the report, Privat Social Club is already under investigation by the Texas Alcoholic Beverage Commission, for an unspecified assault incident that allegedly took place on the premises. 'A female that was fighting with security was 'maced' by security at the location, prior to police arrival. "'Upon arrival, the fight had already been broken prior to police arrival, by security at the location,' SAPD told KENS 5. The San Antonio Police Department said they were called the night of the fight as well," said the report. "Later on in the melee captured on video, it appears that security personnel pepper sprays a different woman. ![]() "That includes four callouts for drunken behavior nine for a fight or assault 12 for a burglary alarm 12 for shots fired, heard or a shooting in progress and 19 for a disturbance, five of those with a gun involved." Bar staff says they hired a private security company called Precision Defense Group to be on the premises, which since November has been the site of various police responses," reported Jeremy Baker. "In the video, a woman can be seen being picked up and slammed to the ground by someone in uniform. A video circulating on social media shows a security guard in San Antonio, Texas slamming a woman onto the pavement outside Privat Social Club near UTSA after a fight broke out at the club's closing last Saturday, reported KENS 5 on Monday. ![]() ![]() Styccobond F44 is a solvent-free, acrylic emulsion adhesive, which provides high bond strength, strong initial tack and a long open time. ![]() Ball’s Styccobond F44 acrylic adhesive to install Polyflor Polysafe Standard PUR heavy-duty vinyl safety flooring. It can also be used over old adhesive residues, without the need to prime subfloors beforehand.Ĭontractors then used F. Its protein-free formulation makes it suitable for use in clinical and biologically sensitive areas, such as hospitals, laboratories and clean rooms. The product is a fast-setting, fast-drying, two-component levelling compound for preparing sound internal subfloors. Ball’s Stopgap 1200 Pro levelling compound was applied to create a perfectly smooth base for the installation of floorcoverings. Stopgap Fill and Prime is a cement-based primer with a thixotropic consistency, meaning it is easily applied by hand but sets to a gel-like consistency on standing, allowing it to fill gaps at joints between panels, preventing depressions resulting from a subsequently-applied levelling compound sinking into them while setting. The upper floor was partitioned to create multiple laboratories. Ball’s dual-purpose Stopgap Fill and Prime and Stopgap 1200 Pro levelling compound to prepare 1,500m² of metal raised access panels over two floors before installing vinyl sheet safety flooring throughout. and the company’s Styccobond F44 acrylic adhesive have been used to create a laboratory-standard flooring finish at a new facility for international laboratories operator Tentamus Group, at Pride Park in Derbyshire.Ĭontractors from JDF Flooring used F. 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![]() Theta Waves, operating at 3.5hz to 7hz, are slow moving and relaxed waves that are produced when sleeping, resting, and dreaming (even daydreaming!) This is why for centuries, Theta Waves have been produced (and used) during meditation, although prior to the discovery of brainwave patterns, people did not know this.Ī common symptom of the brain producing Theta Waves is when one feels like they have entered a trance, feeling like the mind has lost control and left to the will of the cosmos or some higher power, and yet it is still able to recognize the surroundings and the goings-on around. New research has shown that one brainwave type in particular seems to be the happy medium of all three, giving a good balanced dose of the benefits of Alpha, Beta, and Delta Waves: Theta Waves. Read on to discover the immense benefits of Theta brainwaves. Any of the beautifully crafted Theta Gateway tracks will take you into deep Theta the first time and every time you use them.Ģ. So, in total, you can get 49 one-hour meditation tracks and one ten-minute super-boost meditation track.ġ. There are four ambient track mixes per level and is only $47 for a 48-hour, 49-track Theta Gateway Genius Pack (delivered online) containing Theta Gateway (theta wave), Serotonin Release (alpha wave), Quantum Epsilon (very low epsilon wave) and a ten-minute alpha->theta->delta daytime boost meditation bonus track. Try for a month, and if there's any reason at all that you would like a refund, just drop us a line and we'll refund your payment. 1x 10-Minute Alpha-Theta-Delta Daytime Super Boost Spirit Rejuvenation Meditation TrackĪll backed by our 100% No-Questions Asked 30-day money-back guarantee. ![]() 4x 1 hour Quantum Epsilon Meditation Tracks ![]() 4x 1 hour Serotonin Release Meditation Tracks 4x 1 hour Theta Gateway Meditation Tracks Theta Gateway (Platinum, with all of the below described), plus 4 one-hour Delta tracks, 4 one-hour Alpha tracks and 4 one-hour Theta tracks - Also Contains a Transcendental Meditation How-To Guide 2-Page PDF (Transcendental Meditation costs $2000 to learn in a TM Center!)ĭeep Catalyst - A Premium Binaural Beats Meditation Program for Awareness, Healing, Sleep, Study, Stress & Relaxation The Theta Gateway Binaural Beats Brainwave Entrainment Meditation Program Contains 49 beautiful meditation tracks. Theta Gateway (Gold, with all of the below described), plus 4 one-hour Delta tracks and 4 one-hour Alpha tracks Theta Gateway (Silver, with all of the below described), plus 4 one-hour Delta tracks Theta Gateway (Bronze, with all of the below described) ".has worked for my insomnia like nothing else has" - Anthony W Options: " Blissful, relaxing, energizing " - Bradley H, WY, USA ![]() "A great solution for me to what was before a tough meditation practise" - Louise S "This program has helped me to grow stronger emotionally" - Dean S, IN, USA ".these are the most powerful meditation sessions I've ever had" - Tom H, CA, USA And we Guarantee it 100% With Our No-Questions-Asked 30 day Money Back Policy! Some recent testimonials: You will find these artistically-crafted and precision-engineered audio tracks helpful for your quality of life and personal growth, we are very confident of that. This is a Free + Premium Brainwave Entrainment Meditation Program including all of Alpha, Theta, Delta and Epsilon Frequencies. This program is amazing for Awareness, Healing, Sleep, Study, Stress & Relaxation. ![]() ![]()
![]() As with Canadian people, older population in 1988 was less than a fifth percent of the general population and it is expected to increased only by approximately 10 percent in the later year. In the beginning of time, Germany had the highest numbers of aging people at 20.45, with five percent increased in 2000, from then it is predicted to continue to rise in the next 30 years to an estimated 1 percent of the total population in Germany. The highest percentage of old people was in Germany and it is expected to continue in 2030, while the lowest percentage was from the United Kingdom and can be seen over the period of time given. Overall, it is predicted that the aging population in three countries will rise from 1988 to 2030. ![]() The table gives information about the changes in the proportion of population of people who are 65 years old and above in three different countries in the year 1988, 2000 and estimated percentage in 2030. The percentage is forecast to reach 30.42% in Germany, 26.35% in Canada, and 20.35% in the UK. On the contrary, the UK showed relatively little change in the proportion of its population aged 65 plus and recorded at just under 15% in both years.īy 2030, it is expected that the population of people aged 65 and above will increase by an approximate rate of 5.5%in the three countries. The most dominant percentage of older people was in Germany, which is predicted to continue to dominate in 2030, whilst the least dominant proportion can be seen in the UK over the period given.īetween 19, both Germany and Canada went up approximately 5% to reach 25.32% and 20.67% respectively. Overall, the population of elderly people rose and is predicted to rise in all three countries from 1988 to 2030. The table illustrates and gives information about the proportion of the population who are 65 and over in 3 countries( Germany, Canada, and the UK) in 1988, 2000, 2023 and the expected percentage in 2030.
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